Enzyme Assignment

CHM 201 BIOCHEMSTRY

Khan Academy Enzyme Worksheet                                         Name: ______________________

Watch tutorials with links indicated and answer the questions below.

https://www.khanacademy.org/science/biology/energy-and-enzymes/introduction-to-enzymes/v/enzymes

https://www.khanacademy.org/test-prep/mcat/biomolecules/enzyme-structure-and-function/v/enzymes-and-activation-energy

1. What would an energy curve of a spontaneous vs nonspontaneous reaction look like on the graph below? Use arrows to indicate activation energy, D G. (0.5 points)

https://www.khanacademy.org/test-prep/mcat/biomolecules/enzyme-structure-and-function/v/the-induced-fit-model-of-enzyme-catalysis

2. Explain how an induced fit occurs. (Between 4-5 minutes within the video.) (1 point)

https://www.khanacademy.org/test-prep/mcat/biomolecules/enzyme-structure-and-function/v/the-six-types-of-enzymes

3. List the six categories of enzymes. Give one example for one enzyme (one total; not one for each). (1 point)

https://www.khanacademy.org/test-prep/mcat/biomolecules/enzyme-structure-and-function/v/cofactors-coenzymes-and-vitamins

4. How do co-enzymes and co-factors differ? How do vitamins and mineral co-enzymes/cofactors differ? (0.5 points)

https://www.khanacademy.org/test-prep/mcat/biomolecules/enzyme-structure-and-function/v/enzymes-and-their-local-environment

5. What are the two enzymes important in human digestions; where are they present in the body; what are their optimum pH values?(0.5 points)

https://www.khanacademy.org/test-prep/mcat/biomolecules/enzyme-kinetics/v/an-introduction-to-enzyme-kinetics

6. In the overall reaction described E + S <-> ES <-> E + P, what do E, S, ES, and P stand for; explain where S has gone, where P comes from, and why E persists. (0.5 points)

7. On the curve shown of an enzyme reaction, identify on the respective axes where V_max and K_M are located. (0.5 points)

https://www.khanacademy.org/test-prep/mcat/biomolecules/enzyme-kinetics/v/steady-states-and-the-michaelis-menten-equation(middle section is dense; you can skip to about 4:30)

8. What assumptions are made to consider a system at a steady state?(0.5 points)

9. What is the Michaelis-Menton Equation (write out the equation)? (1 point)

10. What is K_M? What are the units of K_M?(0.5 points)

11. How do you measure an enzyme’s catalytic efficiency? (0.5 points)

12. A low K_M compared to a high Km indicates what about the efficiency of an enzyme? (0.5 points)

https://www.medschoolcoach.com/lineweaver-burk-plots-mcat-biochemistry/#:~:text=The%20Lineweaver%20Burk%20plot%20is,called%20the%20double%20reciprocal%20plot

https://www.khanacademy.org/science/ap-biology/cellular-energetics/environmental-impacts-on-enzyme-function/v/noncompetitive-inhibition

https://www.khanacademy.org/science/ap-biology/cellular-energetics/environmental-impacts-on-enzyme-function/v/noncompetitive-inhibition

In addition to the above, please use your textbook to answer the following questions:

13. Why is the Lineweaver-Burke Plot useful in enzyme kinetics? Label the axes and identify the y-intercept on the graph. (1 point)

14. Do competitive inhibitors affect the V_MAX or K_M? What about uncompetitive inhibitors? How do they affect the V_MAXand K_M ?(0.5 points)

15. Increasing substrate concentrations will only be able to overcome the inhibitory effects of which kind of inhibitors? (1 point)

At the end of this exercise, go back to Khan Academy and complete the questions:

Enzyme Structure and Function Questions https://www.khanacademy.org/test-prep/mcat/biomolecules/enzyme-structure-and-function/e/enzyme-structure-and-function-questions

Enzyme Kinetics Questionshttps://www.khanacademy.org/test-prep/mcat/biomolecules/enzyme-kinetics/e/enzyme-kinetics-questions